adenovirus expressing cre recombinase  (Vector Biolabs)

Journal: Frontiers in Oncology

Article Title: Amyloid precursor-like protein 2 expression in macrophages: differentiation and M1/M2 macrophage dynamics

doi: 10.3389/fonc.2025.1570955

Figure Lengend Snippet: APLP2 KO shifts macrophage subpopulations at baseline and post pro-inflammatory stimulation. (A) APLP2-KO mice were generated by breeding C57BL/6 mice expressing cre recombinase housed under the EIIa adenovirus promoter with mice containing floxed Aplp2 . Genotypes of offspring were verified by PCR for Aplp2 and EIIa-cre. (B, C) Bone marrow samples isolated from femurs and tibias of APLP2-WT C57BL/6 mice and APLP2-KO mice were treated with media supplemented with L-929-conditioned media as an M-CSF source. The generated macrophages were left unstimulated as a control ( left ) or stimulated with 10 ng/mL LPS + 20 ng/mL IFN-γ (pro-inflammatory stimuli, 6 hours, middle ) or with 10 ng/mL IL-4 (anti-inflammatory stimuli, 24 hours, right ) for determination of (B) iNOS/Arg1 and (C) CD68/CD206 expression. Results represent mean ± SEM. Statistical significance was determined by unpaired t-test (n=3). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Article Snippet: APLP2-KO mice on the C57BL/6 background were produced by crossing C57BL/6 mice with cre recombinase under the EIIa adenovirus promoter [B6.FVB-Tg(EIIa-cre)C5379Lmgd/J] (The Jackson Laboratory, Bar Harbor, ME, USA) with previously described floxed Aplp2 mice ( ).

Techniques: Generated, Expressing, Isolation, Control